GYIG OpenIR  > 环境地球化学国家重点实验室  > 期刊论文
A reliable compound-specific nitrogen isotope analysis of amino acids by GC-C-IRMS following derivatisation into N-pivaloyl-iso-propyl (NPIP)esters for high-resolution food webs estimation
Zhongyi Zhang JingTian Hongwei Xiao; Nengjian Zheng; Xiaofei Gao; Renguo Zhu; Huayun Xiao
2016
Source PublicationJournal of Chromatography B
Volume1033Issue:2Pages:382-389
Abstract
The signatures of natural stable nitrogen isotopic composition (delta N-15) of individual amino acid (AA) have been confirmed to be a potentially effective tool for elucidating nitrogen cycling and trophic position of various organisms in food webs. In the present study, a two-stage derivatisation approach of esterification followed by acylation was evaluated. The biological samples underwent acid hydrolysis and the released individual AA was derivatived into corresponding N-pivaloyl-isopropyl (NPIP) esters for nitrogen isotopic analysis in gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Usually, 13 individual AA derivatives were separated with fine baseline resolution based on a nonpolar gas chromatography column (DB-5 ms). The minimum sample amount required under the presented conditions is larger than 20 ngN on column in order to accurately determine the delta N-15 values. The delta N-15 values determined by GC-C-IRMS with a precision of better than 1 parts per thousand, were within 1 parts per thousand after empirical correction compared to the corresponding measured by element analysis (EA)-IRMS. Bland-Altman plot showed highly consistency of the delta N-15 values determined by the two measurement techniques. Cation exchange chromatography was applied to remove interfering fraction from the extracts of plant and animal samples and without nitrogen isotope fractionation during the treatment procedure. Moreover, this approach was carried out to estimate the trophic level of various natural organisms in a natural lake environment. Results highly proved that the trophic level estimated via the presented AA method well reflected the actual food web structure in natural environments.
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.gyig.ac.cn/handle/352002/6559
Collection环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文
Recommended Citation
GB/T 7714
Zhongyi Zhang JingTian Hongwei Xiao,Nengjian Zheng,Xiaofei Gao,et al. A reliable compound-specific nitrogen isotope analysis of amino acids by GC-C-IRMS following derivatisation into N-pivaloyl-iso-propyl (NPIP)esters for high-resolution food webs estimation[J]. Journal of Chromatography B,2016,1033(2):382-389.
APA Zhongyi Zhang JingTian Hongwei Xiao,Nengjian Zheng,Xiaofei Gao,Renguo Zhu,&Huayun Xiao.(2016).A reliable compound-specific nitrogen isotope analysis of amino acids by GC-C-IRMS following derivatisation into N-pivaloyl-iso-propyl (NPIP)esters for high-resolution food webs estimation.Journal of Chromatography B,1033(2),382-389.
MLA Zhongyi Zhang JingTian Hongwei Xiao,et al."A reliable compound-specific nitrogen isotope analysis of amino acids by GC-C-IRMS following derivatisation into N-pivaloyl-iso-propyl (NPIP)esters for high-resolution food webs estimation".Journal of Chromatography B 1033.2(2016):382-389.
Files in This Item:
File Name/Size DocType Version Access License
A reliable compound-(857KB) 开放获取LicenseView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhongyi Zhang JingTian Hongwei Xiao]'s Articles
[Nengjian Zheng]'s Articles
[Xiaofei Gao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhongyi Zhang JingTian Hongwei Xiao]'s Articles
[Nengjian Zheng]'s Articles
[Xiaofei Gao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhongyi Zhang JingTian Hongwei Xiao]'s Articles
[Nengjian Zheng]'s Articles
[Xiaofei Gao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A reliable compound-specific nitrogen isotope analysis of amino acids.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.