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Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification
Shuxun Shao;  Xiubo Mi;  Laurent Ouerdane;  Ryszard Lobinski;  Juan Francisco García-Reyes;  Antonio Molina-Díaz;  Andrea Vass;  Mihály Dernovics
2014
发表期刊Food Analytical Methods
卷号7期号:5页码:1147-1157
摘要

Orthogonal liquid chromatographic (ion exchange, reversed phase, and ion pairing) and mass spectrometric [electrospray ionization (ESI)-TOF-MS, ESI-Orbitrap MS, and inductively coupled plasma mass spectrometry (ICPMS)] methods were addressed to identify and quantify selenium species from a naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) sample after proteolytic digestion. While selenomethionine (10.1 mg/kg as Se) and selenate (9.5 mg/kg as Se) could be quantified in a straightforward way by anion exchange LC-ICP-MS technique, a multistep purification protocol was required to identify Se-methylselenocysteine and γ-glutamyl-Se-methylselenocysteine in an unambiguous way prior to quantification by using either in-source fragmentation (LC-ESI-TOF-MS) or collision-induced dissociation (LC-ESI-Orbitrap MS). Finally, Se -methylselenocysteine (2.6 mg/kg as Se) and γ-glutamyl-Se-methylselenocysteine (1.2 mg/kg as Se) could contribute to the overall selenium recovery of 72 %. This sample is the first of the Faboideae subfamily and Phaseolus ssp. to be speciated to such an extent for selenium including γ-glutamyl-Se-methylselenocysteine, a highly potential selenium species, which makes this bean material an ideal candidate for functional food purposes

关键词Selenium speciation orbitrap phaseolus γ-glutamyl-se-methylselenocysteine
收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.gyig.ac.cn/handle/42920512-1/11199
专题矿床地球化学国家重点实验室
作者单位1.State Key Laboratory of Ore Deposit Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, 46 Guanshui Road, Guiyang 550002, China
2.College of Earth Science, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China
3.Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, UMR 5254, CNRS/UPPA, Hélioparc, 2 av. Pierre Angot, 64053 Pau, France
4.Analytical Chemistry Research Group, Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas, Edif B3, 23071 Jaén, Spain
5.Department of Applied Chemistry, Corvinus University of Hungary, Villányi út 29-33, 1118 Budapest, Hungary
6.Department of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland
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GB/T 7714
Shuxun Shao;Xiubo Mi;Laurent Ouerdane;Ryszard Lobinski;Juan Francisco García-Reyes;Antonio Molina-Díaz;Andrea Vass;Mihály Dernovics. Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification[J]. Food Analytical Methods,2014,7(5):1147-1157.
APA Shuxun Shao;Xiubo Mi;Laurent Ouerdane;Ryszard Lobinski;Juan Francisco García-Reyes;Antonio Molina-Díaz;Andrea Vass;Mihály Dernovics.(2014).Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification.Food Analytical Methods,7(5),1147-1157.
MLA Shuxun Shao;Xiubo Mi;Laurent Ouerdane;Ryszard Lobinski;Juan Francisco García-Reyes;Antonio Molina-Díaz;Andrea Vass;Mihály Dernovics."Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification".Food Analytical Methods 7.5(2014):1147-1157.
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