In utero exposure to methylmercury impairs cognitive function in adult offspring: Insights from proteomic modulation | |
Wenjuan Wang; Li Zhang; Caiyun Deng; Fang Chen; Qing Yu; Yi Hu; Qin Lu; Ping Li![]() | |
2022 | |
Source Publication | Ecotoxicology and Environmental Safety
![]() |
Volume | 231Pages:113191 |
Abstract | Methylmercury (MeHg) is a hazardous substance that has unique neurodevelopmental toxic effects. However, its molecular alteration profile, sensitive response biomarkers, and mechanism of neuronal injury remain largely unknown. Here, the effects of intrauterine methylmercury chloride (low-, medium- and high-dose groups: 0.6 mg/kg/d, 1.2 mg/kg/d, 2.4 mg/ kg /d, respectively) exposure on learning and memory were assessed in offspring rats by behavioral tests, pathological analysis and hippocampal proteomic analysis. The results suggested that intrauterine MeHg exposure impairs spatial learning and memory and leads a significant reduction in the number and dispersion scattered arrangement in the hippocampus of offspring. Furthermore, in the tandem mass tag-based proteomics analysis, compared with the control group, a total of 74 differentially expressed proteins (DEPs) were found in the MeHg exposure groups; specifically, 32 down-regulated and 42 up-regulated proteins were identified. In addition, the pathways enrichment analysis indicated that these DEPs are implicated in several biological processes, such as synaptic plasticity and energy metabolism, as well as various molecular functional categories. Simultaneously, MeHg reduced the postsynaptic density, diminished the active zone, amplified the synaptic cleft and changed the synaptic interface of pyramidal cells. Western blot analysis further revealed that MeHg significantly reduced the levels of Forkhead box protein (FOXP2), Synaptophysin (SYP) and Postsynaptic density protein 95 (PSD-95), and down-regulated the N-methyl-D-aspartate receptor 1 (NMDAR1), N-methyl-D-aspartate receptor 2 A (NR2A) and N-methyl-D-aspartate receptor 2B (NR2B). In general, from a functional perspective, most overlapping proteins were related to NMDA receptor-mediated glutamatergic signaling, which is an excitotoxicity mechanism known to influence learning and memory. These discoveries contribute to our understanding of the relationship between MeHg and cognitive deficits and provide insight into the protein mediators of this relationship and possible prospective early biomarkers. |
Keyword | Methylmercury hippocampus memory Impairments rat offspring proteomic |
DOI | 10.1016/j.ecoenv.2022.113191 |
URL | 查看原文 |
Indexed By | SCI |
Language | 英语 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.gyig.ac.cn/handle/42920512-1/13539 |
Collection | 环境地球化学国家重点实验室 |
Affiliation | 1.The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 550025, Guizhou, PR China 2.State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, PR China |
Recommended Citation GB/T 7714 | Wenjuan Wang,Li Zhang,Caiyun Deng,et al. In utero exposure to methylmercury impairs cognitive function in adult offspring: Insights from proteomic modulation[J]. Ecotoxicology and Environmental Safety,2022,231:113191. |
APA | Wenjuan Wang.,Li Zhang.,Caiyun Deng.,Fang Chen.,Qing Yu.,...&Aihua Zhang.(2022).In utero exposure to methylmercury impairs cognitive function in adult offspring: Insights from proteomic modulation.Ecotoxicology and Environmental Safety,231,113191. |
MLA | Wenjuan Wang,et al."In utero exposure to methylmercury impairs cognitive function in adult offspring: Insights from proteomic modulation".Ecotoxicology and Environmental Safety 231(2022):113191. |
Files in This Item: | There are no files associated with this item. |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment